TRALI investigations and neutrophil genotyping services

For additional information and order forms, go to: www.redcrosslab.org

Transfusion Related Acute Lung Injury (TRALI) Investigations

TRALI, the most frequent cause of transfusion-related deaths in the United States, has been associated with antibodies against neutrophils, HLA Class I and/or HLA Class II. The American Red Cross Neutrophil Reference Laboratory offers TRALI antibody investigations, including neutrophil and HLA antibody detection and neutrophil antigen typing. Medical consultations with experienced clinicians and highly skilled immunohematology and serology experts are also available. The Neutrophil Reference Laboratory is participating in a definitive TRALI study funded by the National Institutes of Health, and is an international leader in neutrophil serology analysis.

Neutrophil Genotyping by SSP-PCR
(Sequence Specific Primers-Polymerase Chain Reaction)

The American Red Cross Neutrophil Reference Laboratory uses DNA molecular technology to determine the presence of the three polymorphisms associated with the HNA-1 antigen system: HNA-1a (NA1), HNA-1b (NA2), and HNA-1c (SH). The probability of future children being affected with neonatal alloimmune neutropenia can be estimated by genotyping DNA. Genotyping can be used to confirm antibody specificity identified by serologic methods. Neutrophil genotyping by SSP-PCR is considered the gold-standard in genotyping. Its benefits include:

• Clients can submit a much smaller specimen volume with less stringent sample and shipping requirements.
• Genomic DNA can be isolated from all nucleated cells; even patients with severe neutropenia can be typed for these alleles.
• Isolated DNA can be stored frozen and available for retyping at a later date

For more information contact 1-800-652-9742, or visit: www.redcrosslab.org.